ENTER


Полный размерЗакрыть

The expression level of the growth factors (VEGF and Ang-1) and cytokines (IL-10 and TNF-alfa) in conjunctival biopsy specimens patients with type 2 diabetes with nonproliferative diabetic retinopathy (NPDR)

Details
Written by Super User
Category: #2 (07) 2017
Published: 17 April 2018
Hits: 2393

https://doi.org/10.30702/Ophthalmology.2017/07.art4

 Vitovskaya O. P.1, Taha Salah Ahmad1, Klimenko P. P.2


1Bogomolets National Medical University, Kyiv, Ukraine
2The State Institution “Institute of Gerontology of them D. F. Chebotareva” Academy of Medical Sciences of Ukraine, Kyiv, Ukraine

Summary. In the present study, the analysis of gene activity of VEGF, TNF-alfa, Ang-1, IL-10 in mucosal biopsies of the conjunctiva of the eyes of diabetic patients – type 2, with nonproliferative diabetic retinopathy (NPDR). The study included 55 patients (110 eyes) who were divided into two groups: the first control group consisted of 20 patients (40 eyes); the second – patients with type 2 diabetes with NPDR comprised 35 patients (70 eyes). The obtained results in the biopsy of the conjunctiva of the eyes of diabetic patients – type 2, with nonproliferative diabetic retinopathy (NPDR) testify to the increase in the activity of genes of VEGF, TNF-alfa, participating in the processes involved in angiogenesis and inflammation, and the inhibition of the activity of genes Ang-1, IL-10, contribute to the stabilization of the structure and functioning of the vascular wall and inhibiting inflammatory processes.

 Keywords: diabetes mellitus type 2, non-proliferative diabetic retinopathy, VEGF – vascular endothelial growth factor, Ang-1 – angiopoietin-1, IL-10 – interleukin-10, TNFalfa – tumour necrosis factor-alpha, HIF-1 – inducible hypoxia factor-1.

REFERENCES

  1. Poveshchenko АF, Konenkov VI. [Mechanisms and Factors of Angiogenesis]. Uspyekhi fiziologichyeskikh nauk. 2010;41(2):68–89. (in Russian).
  2. Halvorsen B, Wæhre T, Scholz H, Damås JK, Yndestad A, Aukrust P. Role of interleukin-10 in atherogenesis and plaque stabilization. Future Cardiology. 2006 Jan;2(1):75–83.
  3. Chen JX, Stinnett A. Disruption of Ang-1/Tie-2 signaling contributes to the impaired myocardial vascular maturation and angiogenesis in type II diabetic mice. Arterioscler Thromb Vasc Biol. 2008;28(9):1606–13.
  4. Costagliola С, Romano V, De Tollis M, Aceto F, dell’Omo R, Romano MR, Pedicino C, Semeraro F. TNF-Alpha Levels in Tears: A Novel Biomarker to Assess the Degree of Diabetic Retinopathy. Mediators of Inflammation. 2013;2013:1–6.
  5. Graves DT, Kayal RA. Diabetic complications and dysregulated innate immunity. Front Biosci. 2008;1(13):1227–39.
  6. Haddad JJ, Fahlman CS. Redox- and oxidant-mediated regulation of interleukin-10: an antiinflammatory, antioxidant cytokine? Biochemical and Biophysical Research Communications. 2002;297:163–76.
  7. Maloney JP, Gao L. Proinflammatory Cytokines Increase Vascular Endothelial Growth Factor Expression in Alveolar Epithelial Cells. Mediators of Inflammation. 2015;2015:1–7.
  8. Ohba T, Haro H, Ando T, Wako M, Suenaga F, Aso Y, Koyama K, Hamada Y, Nakao A. TNF-alpha-induced NF-kappaB signaling reverses age-related declines in VEGF induction and angiogenic activity in intervertebral disc tissues. J Orthop Res. 2009;27(2):229–35.
  9. Osaadon P, Fagan XJ, Lifshitz T, Levy J. A review of anti-VEGF agents for proliferative diabetic retinopathy. Eye (Lond). 2014;28(5):510–20.
  10. Thurston G, Rudge JS, Ioffe E, Zhou H, Ross L, Croll SD, Glazer N, Holash J, McDonald DM, Yancopoulos GD. Angiopoietin-1 protects the adult vasculature against plasma leakage. Nat Med. 2000;6(4):460–63.
  11. Unwin N, Whiting D, Guariguata L, Ghyoot G, Gan D. IDF Diabetes atlas. 5th ed. International Diabetes federation; 2011. 137 р.
  12. Wang X, Wang G, Wang Y. Intravitreous vascular endothelial growth factor and hypoxiainducible factor 1a in patients with proliferative diabetic retinopathy. Am J Ophthalmol. 2009;148(6):883–889.
  13. Yaghini N, Mahmoodi M, Asadikaram Gh R, Hassanshahi GhH, Khoramdelazad H, Kazemi Arababadi M. Serum Levels of Interleukin 10 (IL-10) in Patients with Type 2 Diabetes. Iran Red Crescent Med J. 2011;13(10):752.
  14. Yoshida S, Yoshida A, Ishibashi T. Induction of IL-8, MCP-1, and bFGF by TNF-alpha in retinal glial cells: implications for retinal neovascularization during post-ischemic inflammation. Graefes Arch Clin Exp Ophthalmol. 2004;242(5):409–13.
  15. Yau JW, Rogers SL, Kawasaki R, Lamoureux EL, Kowalski JW, Bek T, Chen SJ, Dekker JM, Fletcher A, Grauslund J, Haffner S, Hamman RF, Ikram MK, Kayama T, Klein BE, Klein R, Krishnaiah S, Mayurasakorn K, O’Hare JP, Orchard TJ, Porta M, Rema M, Roy MS, Sharma T, Shaw J, Taylor H, Tielsch JM, Varma R, Wang JJ, Wang N, West S, Xu L, Yasuda M, Zhang X, Mitchell P, Wong TY. Meta-Analysis for Eye Disease (META-EYE) Study Group. Global prevalence and major risk factors of diabetic retinopathy. Diabetes Care. 2012;35(3):556–64.

Received: 30 May 2017

Published: December 2017