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The expression level of the growth factors (VEGF and Ang-1) and cytokines (IL-10 and TNF-alfa) in conjunctival biopsy specimens patients with type 2 diabetes with nonproliferative diabetic retinopathy (NPDR)

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Category: #2 (07) 2017
Published: 17 April 2018
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https://doi.org/10.30702/Ophthalmology.2017/07.art4

 Vitovskaya O. P.1, Taha Salah Ahmad1, Klimenko P. P.2


1Bogomolets National Medical University, Kyiv, Ukraine
2The State Institution “Institute of Gerontology of them D. F. Chebotareva” Academy of Medical Sciences of Ukraine, Kyiv, Ukraine

Summary. In the present study, the analysis of gene activity of VEGF, TNF-alfa, Ang-1, IL-10 in mucosal biopsies of the conjunctiva of the eyes of diabetic patients – type 2, with nonproliferative diabetic retinopathy (NPDR). The study included 55 patients (110 eyes) who were divided into two groups: the first control group consisted of 20 patients (40 eyes); the second – patients with type 2 diabetes with NPDR comprised 35 patients (70 eyes). The obtained results in the biopsy of the conjunctiva of the eyes of diabetic patients – type 2, with nonproliferative diabetic retinopathy (NPDR) testify to the increase in the activity of genes of VEGF, TNF-alfa, participating in the processes involved in angiogenesis and inflammation, and the inhibition of the activity of genes Ang-1, IL-10, contribute to the stabilization of the structure and functioning of the vascular wall and inhibiting inflammatory processes.

 Keywords: diabetes mellitus type 2, non-proliferative diabetic retinopathy, VEGF – vascular endothelial growth factor, Ang-1 – angiopoietin-1, IL-10 – interleukin-10, TNFalfa – tumour necrosis factor-alpha, HIF-1 – inducible hypoxia factor-1.

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Received: 30 May 2017

Published: December 2017