ENTER


Полный размерЗакрыть

Research on enzymes activities by the cycle of Krebs in eye's tissue at experimental diabetic retinopathy

Details
Written by Super User
Category: #1 (08) 2018
Published: 29 August 2018
Hits: 2313


https://doi.org/10.30702/Ophthalmology.2018/08.02

Semenko V. V.1, Serdiuk V. M.1, 2, Savytskyi I. V.3


1Dnipropetrovsk Regional Clinical Ophthalmology Hospital, Dnipro, Ukraine
2Dnipropetrovsk Medical Academy of Health Ministry of Ukraine, Dnipro, Ukraine
3Odessa national medical university, Odesa, Ukraine

Resume. Modelling of type 1 diabetes mellitus (DMТ1) is the basis for the new methods development for diabetic retinopathy treatment.

Goal. The goal of the study was the activity of succinate dehydrogenase (SDG), lactate dehydrogenase (LDG) and malate dehydrogenase (MDG) research to assess the energy metabolism condition in eyeball tissues with a background of a DMТ1 developed model.

Materials and methods. Diabetes was simulated with a triple intraperitoneal injection of alloxan at dose 7.5 ml / 200 g of animal weight. Introduction of alloxan was carried out with the background of free access to 5 % fructose solution.

Results. In the ganglionic layer differences in specific weight of animals were revealed in LDG activity analysis; differences in the activity of MDG, LDG, and SDG were revealed by high and very high enzyme activity classes. In the granular layer, differences were found between the group with the DMТ1 model and the control group in the specific weight of animals in SDG and LDG activity analysis. In the vascular layer, SDG and LDG activity differences were found in high and moderate activity class, and in very high enzymatic activity class in MDG and LDG activity analysis.

Conclusions. The obtained results indicate that during early stages of DMТ1 development there are significant changes in bioenergetics processes, which indicates the necessity to include in the debut of the disease metabolic profile drugs.

Keywords: diabetes mellitus, alloxan model, diabetic retinopathy, oxidation-reduction enzymes.

REFERENCES

  1. Prudius PG, Bondarchuk NV, Semenjuk ІV. [Prevalence of diabetic retinopathy among patients with type 1 diabetes mellitus in the Vinnytsia region]. Mіzhnarodnyi endokrynologіchnyi zhurnal. 2007;2:44–6. (in Ukrainian).
  2. Velykyi M, Burda V, Biront N. [Effect of nicotinamide on the activity of antioxidant defense enzymes in experimental diabetes]. Ukr.Biochem.J. 1996;2:109–14 (in Ukrainian).
  3. Shymanskyi I, Kuchmerovska T, Donchenko G. [Correction of nicotinoyl-GABA oxidative stress with diabetic neuropathy]. Ukr.Biochem.J. 2002;5:89–95. (in Ukrainian).
  4. Charron MJ, Bonner-Weir S. Implicating PARP and NAD depletion in type I diabetes. Nat Med. 1999;3:269–70.
  5. Kamilov R. [Mitochondria as a model for the evaluation of pathological mechanisms under the influence of chemical pollutants widely used in the petrochemical and oil refining industry]. Vyatskiy meditsinskiy vestnik. 2007;4:108–11. (in Russian).
  6. Lojda Z, Gossrau R, Schiebler TH. Enzyme Histochemistry: A Laboratory Manual. Raykhlin NT, editor. Moscow: Mir;1982. (in Russian).
  7. Nasibullin BA. [Histochemically revealed changes of enzymes CTA activity in neurones of rat brain sensomotoric cortex in the dynamics of hypokinesia]. Aktualni problemy transportnoi medytsyny. 2006;4:126–32. (in Russian).
  8. Shapiro SS, Wilk MB. An analysis of variance test for normality (complete samples). Biometrika. 1956;52(3–4):591–611.
  9. Polyakov IV, Sokolova NS. Practical guide on medical statistics. Moscow: Meditsina; 1975. (in Russian).
  10. Eprincev AT, Shevchenko MJu, Popov VN. [Carbohydrate metabolism in liver of rats with food deprivation and experimental diabetes]. Izvestiya RAN, seriya biologicheskaya. 2008;1:115–8. (in Russian).
  11. Popov EM. Structural and functional organization of proteins. Moscow: Science; 1992. (in Russian).
  12. Budancev AJu. Fundamentals of histochemistry: Textbook [Internet]. Pushchino: Pushhinskij gos. un-t; 2008 [cited 2008 Jul 9]. Available at: http://window.edu.ru/resource/159/ 59159. (in Russian).
  13. Skulachev VP. [Evolution, mitochondria and oxygen]. Sorosovskiy obrazovatelnyy zhurnal. 1999;9:1–7. (in Russian).
  14. Maevskiǐ EI, Grishina EV, Rozenfel'd AS, Ziakun AM, Vereshchagina VM, Kondrashova MN. [Anaerobic formation of succinate and facilitation of its oxidation - possible mechanisms of cell adaptation to oxygen defciency]. Biofzika. 2000;3: 509–13. (in Russian).
  15. Ashastin BV. [Possibilities of maintaining the mitochondrial apparatus during hypoxia by substrates of energy metabolism]. Vestnik JuUrGU. 2012;42:114–8. (in Russian).
  16. Pearse AGE. Histochemistry: Theoretical and Applied. Moscow: Foreign literature; 1962. (in Russian).

Received: 11 Apr. 2018

Published: September 2018