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Research on enzymes activities by the cycle of Krebs in eye's tissue at experimental diabetic retinopathy

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Category: #1 (08) 2018
Published: 29 August 2018
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https://doi.org/10.30702/Ophthalmology.2018/08.02

Semenko V. V.1, Serdiuk V. M.1, 2, Savytskyi I. V.3


1Dnipropetrovsk Regional Clinical Ophthalmology Hospital, Dnipro, Ukraine
2Dnipropetrovsk Medical Academy of Health Ministry of Ukraine, Dnipro, Ukraine
3Odessa national medical university, Odesa, Ukraine

Resume. Modelling of type 1 diabetes mellitus (DMТ1) is the basis for the new methods development for diabetic retinopathy treatment.

Goal. The goal of the study was the activity of succinate dehydrogenase (SDG), lactate dehydrogenase (LDG) and malate dehydrogenase (MDG) research to assess the energy metabolism condition in eyeball tissues with a background of a DMТ1 developed model.

Materials and methods. Diabetes was simulated with a triple intraperitoneal injection of alloxan at dose 7.5 ml / 200 g of animal weight. Introduction of alloxan was carried out with the background of free access to 5 % fructose solution.

Results. In the ganglionic layer differences in specific weight of animals were revealed in LDG activity analysis; differences in the activity of MDG, LDG, and SDG were revealed by high and very high enzyme activity classes. In the granular layer, differences were found between the group with the DMТ1 model and the control group in the specific weight of animals in SDG and LDG activity analysis. In the vascular layer, SDG and LDG activity differences were found in high and moderate activity class, and in very high enzymatic activity class in MDG and LDG activity analysis.

Conclusions. The obtained results indicate that during early stages of DMТ1 development there are significant changes in bioenergetics processes, which indicates the necessity to include in the debut of the disease metabolic profile drugs.

Keywords: diabetes mellitus, alloxan model, diabetic retinopathy, oxidation-reduction enzymes.

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Received: 11 Apr. 2018

Published: September 2018