Gaydamaka T. B.¹, Rafalyuk S. Ya.²

¹GI “The Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine”, Odesa, Ukraine
²Danylo Halytsky Lviv National Medical University, Lviv, Ukraine

 Abstract

Introduction. In today’s world the problem of the syndrome of dry eye is quite relevant and meaningful in ophthalmology.

 Purpose. The aim is to study the influence of Lipoflavon redox enzymes in the tear fluid of patients with keratitis and dry eye syndrome.

 Methods. Clinical and biochemical studies have been conducted on 84 patients with dry eye syndrome and keratitis. Patients were divided into 4 groups: group 1 – patients with dry eye syndrome (30 patients), group 2 – patients with herpetic keratitis (17 patients), group 3 – patients with herpetic keratitis and dry eye syndrome (23 patients), group 4 – patients with herpetic keratitis and dry eye syndrome, which use of Lipoflavon (14 patients). In the tear fluid of patients was measured activity of lactate dehydrogenase and malate dehydrogenase.

 Results. Overall analysis of the data suggests that the development of keratitis, patients with dry eye syndrome leads to sharper pathochemical changes in the tear fluid, expressed in significantly higher decrease in the activity of lactate dehydrogenase and malate dehydrogenase as compared with patients with keratitis, which did not have dry eye syndrome. Lipoflavon has a beneficial effect – it reduces the degree of impairment of biochemical parameters in the tear fluid of patients with keratitis in the presence of dry eye syndrome.

 Conclusions. Patients with dry eye syndrome revealed a clear damage to cellular and subcellular structures of the corneal epithelium, as evidenced by a decrease in activity of the cytosolic enzyme (lactate dehydrogenase) – by 50.7 % and mitochondrial (malate dehydrogenase) – by 41.8 % in the tear fluid of patients. The activity of lactate dehydrogenase and malate dehydrogenase in the tear fluid in the development of keratitis, patients with dry eye syndrome was significantly decreased as compared to the patients who developed keratitis without dry eye syndrome. It was established that Lipoflavon appreciably reduces the negative membranotropic impact of dry eye syndrome in the corneal epithelium, which is clearly expressed when keratitis in patients with dry eye syndrome.

 Keywords: dry eye syndrome, keratitis, cornea, lacrimal fluid, lactate dehydrogenase, malate dehydrogenase. 

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 Received: 4 Feb. 2016

Published: July 2016