Yurevych V.R.
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine

SUMMARY

Introduction. Relevant is the search of drugs aimed at the normalization of metabolic processes in the tissues of the eye in diabetes and hypertension.

Purpose. Examine the condition of the thiol in the retina and optic nerve for modeling open-angle glaucoma (hypertension) in animals with streptozotocin diabetes.

Methods. Studies were conducted on 34 rabbits. Experimental animals were divided into four groups: the first – the control group (10 rabbits), the second – experimental group, animals with diabetes in conditions of hypertension (8 rabbits), the third – the experimental group, animals with diabetes (8 rabbits), the fourth – the experimental group animals with hypertension (8 rabbits). In isolated tissues of the retina and the optic nerve was performed determination of the oxidized, reduced glutathione, as well as the level of thiol and disulfide groups of proteins.

Results. These disorders thiol status of neural tissue in the simulation open angle glaucoma diabetic animals reflect a high degree of oxidative stress and the reduction potential of the reducing and detoxification system of glutathione in the tissue of the optic nerve and retina.

Conclusion. In terms of modeling the experimental glaucoma (hypertension) in animals with streptozotocin diabetes showed a sharp drop in glutathione redox potential of the system in the neural cells of the body. The development of experimental hypertension in diabetic animals accompanied by a reduction of the thiol groups of 34.5 %, and increasing disulfide bond content of 29.7 %. These disorders thiol status of neural tissue in the simulation open angle glaucoma diabetic animals reflect a high degree of oxidative stress and the reduction potential of the reducing and detoxification system of glutathione in the tissue of the optic nerve and retina.

Keywords: hypertension, diabetes, glutathione, thiol, disulfide groups, proteins.

REFERENCES
1. Anyna E. A., Martoplyas K. V. Glaucoma in the adult population of Ukraine. Proceedings of the Filatovski chytannia: naukovo-praktychna konferentsiia oftalmolohiv z mizhnarodnoiu uchastiu. Odessa, 2009, 80–81 (in Russian).
2. Gazirova I. R. Status redox system in patients with primary open-angle glaucoma. Kazanskiy meditsinskiy zhurnal [Kazan Journal of Medicine]. 2012; (93): 53–57 (in Russian). 3. Urich V. P., Tumanov V. P., Panushkina L. A. Glaucoma and neurodegenerative disease. Glaukoma [Glaucoma]. 2012; (1): 62–68 (in Russian).
4. Efimov A., Skorobonskaya N., Zuev N. Diabetic neuropathy. Liky Ukraine [Drugs of Ukraine]. 2005; (3): 21–25 (in Russian).
5. Kashintseva L. T. Role of metabolic disorders in the pathogenesis of glaucoma in the disorder insular apparatus. Oftalmologicheskiy zhurnal [Journal of Ophthalmology]. 1970; (7): 531–536 (in Russian).
6. Kravchuk E. A. Role of free radical oxidation in the pathogenesis of eye diseases. Vestnik oftalmologii [Herald of Ophthalmology]. 2004; (5): 48–51 (in Russian).
7. Nasledov A. SPSS computer data analysis in psychology and social sciences. Saint Petersburg: Piter, 2005, 416 p. (in Russian).
8. Olejnik T. Possibility of correction of glutathione levels in the retina during the development of streptozotocin diabetes. Oftalmologicheskiy zhurnal [Journal of Ophthalmology]. 2007; (3): 68–70 (in Russian).
9. Serdyuk V. N. Thiol-disulfide status of proteins of the retina and optic nerve in the development of experimental glaucoma. Problemy ekolohichnoi ta medychnoi henetyky i klinichnoi imunolohii [Problems of Ecological and Medical Genetics and Clinical Immunology]. 2011; (108): 239–245 (in Russian).
10. Serdyuk V. N. Study of glutathione redox potential in the retina and optic nerve for modeling openangle glaucoma in rabbits. Tavricheskiy mediko-biologicheskiy vestnik [Tauride Medical and Biological Bulletin]. 2011; (53): 142–145 (in Russian).
11. Barber A. J. A new view of diabetic retinopathy: a neurodegenerative disease of the eye. Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2003; (27): 283–290.
12. Izzotti A., Bagnis A., Sacca S. C. The role of oxidative stress in glaucoma. Mutation Research. 2006; (612): 105–114.
13. Kedar P., Chakrabarti C. H. Effect of Jambolan seed treatment on blood sugar lipids and urea in streptozotocine induced diabetes in rabbits. Indian Journal of Physiology and Pharmacology. 1983; (27): 135–140.
14. Lambiase A., Centofanti M., Micera A. Nerve growth factor (NGF) reduces and NGF antibody exacerbates retinal damage induced in rabbit by experimental ocular hypertension. Graefe’s Archive for Clinical and Experimental Ophthalmology. 1997; (235): 780–785.
15. McBean G. J., Aslan M. Thiol redox homeostasis in neurodegenerative disease. Redox Biology. 2015; (1): 26–29.
16. Moreno M. C., Campanelli J., Sande P. Retinal oxidative stress induced by high intraocular pressure. Free Radical Biology and Medicine. 2004; (37): 803–812.
17. Park M. H. Circulating total glutathione in normal tension glaucoma patients: comparison with normal control subjects. Korean Journal of Ophthalmology. 2012; (26): 84–91.
18. Wang J., Wan R., Mo Y. Creating a long-term diabetic rabbit model. Experimental Diabetes Research. 2010; (6): 1–10.
19. Zhu M. D., Cai F. Y. Development of experimental chronic intraocular hypertension in the rabbit. Australian and New Zealand Journal of Ophthalmology. 1992; (20): 225–234.