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1 (08) 2018

Research on enzymes activities by the cycle of Krebs in eye's tissue at experimental diabetic retinopathy

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Category: #1 (08) 2018
Published: Wednesday, 29 August 2018 11:36
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https://doi.org/10.30702/Ophthalmology.2018/08.02

Semenko V. V.1, Serdiuk V. M.1, 2, Savytskyi I. V.3


1Dnipropetrovsk Regional Clinical Ophthalmology Hospital, Dnipro, Ukraine
2Dnipropetrovsk Medical Academy of Health Ministry of Ukraine, Dnipro, Ukraine
3Odessa national medical university, Odesa, Ukraine

Resume. Modelling of type 1 diabetes mellitus (DMТ1) is the basis for the new methods development for diabetic retinopathy treatment.

Goal. The goal of the study was the activity of succinate dehydrogenase (SDG), lactate dehydrogenase (LDG) and malate dehydrogenase (MDG) research to assess the energy metabolism condition in eyeball tissues with a background of a DMТ1 developed model.

Materials and methods. Diabetes was simulated with a triple intraperitoneal injection of alloxan at dose 7.5 ml / 200 g of animal weight. Introduction of alloxan was carried out with the background of free access to 5 % fructose solution.

Results. In the ganglionic layer differences in specific weight of animals were revealed in LDG activity analysis; differences in the activity of MDG, LDG, and SDG were revealed by high and very high enzyme activity classes. In the granular layer, differences were found between the group with the DMТ1 model and the control group in the specific weight of animals in SDG and LDG activity analysis. In the vascular layer, SDG and LDG activity differences were found in high and moderate activity class, and in very high enzymatic activity class in MDG and LDG activity analysis.

Conclusions. The obtained results indicate that during early stages of DMТ1 development there are significant changes in bioenergetics processes, which indicates the necessity to include in the debut of the disease metabolic profile drugs.

Keywords: diabetes mellitus, alloxan model, diabetic retinopathy, oxidation-reduction enzymes.

REFERENCES

  1. Prudius PG, Bondarchuk NV, Semenjuk ІV. [Prevalence of diabetic retinopathy among patients with type 1 diabetes mellitus in the Vinnytsia region]. Mіzhnarodnyi endokrynologіchnyi zhurnal. 2007;2:44–6. (in Ukrainian).
  2. Velykyi M, Burda V, Biront N. [Effect of nicotinamide on the activity of antioxidant defense enzymes in experimental diabetes]. Ukr.Biochem.J. 1996;2:109–14 (in Ukrainian).
  3. Shymanskyi I, Kuchmerovska T, Donchenko G. [Correction of nicotinoyl-GABA oxidative stress with diabetic neuropathy]. Ukr.Biochem.J. 2002;5:89–95. (in Ukrainian).
  4. Charron MJ, Bonner-Weir S. Implicating PARP and NAD depletion in type I diabetes. Nat Med. 1999;3:269–70.
  5. Kamilov R. [Mitochondria as a model for the evaluation of pathological mechanisms under the influence of chemical pollutants widely used in the petrochemical and oil refining industry]. Vyatskiy meditsinskiy vestnik. 2007;4:108–11. (in Russian).
  6. Lojda Z, Gossrau R, Schiebler TH. Enzyme Histochemistry: A Laboratory Manual. Raykhlin NT, editor. Moscow: Mir;1982. (in Russian).
  7. Nasibullin BA. [Histochemically revealed changes of enzymes CTA activity in neurones of rat brain sensomotoric cortex in the dynamics of hypokinesia]. Aktualni problemy transportnoi medytsyny. 2006;4:126–32. (in Russian).
  8. Shapiro SS, Wilk MB. An analysis of variance test for normality (complete samples). Biometrika. 1956;52(3–4):591–611.
  9. Polyakov IV, Sokolova NS. Practical guide on medical statistics. Moscow: Meditsina; 1975. (in Russian).
  10. Eprincev AT, Shevchenko MJu, Popov VN. [Carbohydrate metabolism in liver of rats with food deprivation and experimental diabetes]. Izvestiya RAN, seriya biologicheskaya. 2008;1:115–8. (in Russian).
  11. Popov EM. Structural and functional organization of proteins. Moscow: Science; 1992. (in Russian).
  12. Budancev AJu. Fundamentals of histochemistry: Textbook [Internet]. Pushchino: Pushhinskij gos. un-t; 2008 [cited 2008 Jul 9]. Available at: http://window.edu.ru/resource/159/ 59159. (in Russian).
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  14. Maevskiǐ EI, Grishina EV, Rozenfel'd AS, Ziakun AM, Vereshchagina VM, Kondrashova MN. [Anaerobic formation of succinate and facilitation of its oxidation - possible mechanisms of cell adaptation to oxygen defciency]. Biofzika. 2000;3: 509–13. (in Russian).
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Received: 11 Apr. 2018

Published: September 2018

The results of polytetrafluoroethylene implant application in musculoskeletal stump formation for patients after eye evisceration caused by injuries and related uveitis

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Category: #1 (08) 2018
Published: Tuesday, 28 August 2018 11:25
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https://doi.org/10.30702/Ophthalmology.2018/08.01 

Bigun N.1, Maletskyy A.2


1The Lviv District Hospital, Lviv, Ukraine
2The Filatov Institute of Eye Diseases and Tissue Therapy, Odesa, Ukraine

Summary. The work represents the eviscerating enucleation results of 34 patients with injuries and vasoconstrictive uveitis, which was performed for the musculoskeletal stump (MS) formation for the polytetrafluoroethylene implant. During the observations from 1 to 8 years, all patients achieved a stable effect in the MS formation and there was no noticed tendency to implant resorption.

There was no case of implant denudation after the evisceration due to the chronic inflammatory process. This fact allows concluding that the scleral membrane prevents the regenerative processes violation in the subconjunctival space and conjunctiva.

The positive side of the polytetrafluoroethylene implant includes its suitability for simulation, which allows further corrections during surgery.

The advantage of the method is nasal membranes usage for the prosthesis, which prevents the implant from infections. The developed technology of the eye prosthesis rigid fixation with the MS allowed improving its total mobility in four meridians from 115º to 135º (median 130º).

Keywords: implant, polytetrafluoroethylene, eye injuries, uveitis, musculoskeletal stump.

REFERENCES

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  2. Galimova VU. [Influence of the cosmetic effect of eye prosthetics on the quality of life of patients with anophthalmia, atrophy and subatrophy of the eyeball]. Refraktsionnaya khirurgiya i oftalmologiya. 2004;1:27–9. (in Russian).
  3. Grishina NI, Fedorischeva LE, Kolbenev IO. Traumatic orbital injuries, the methods of diagnosis and treatment. In: Brovkina A, editor. Collection of research papers “Diseases, tumors and traumatic injuries of the orbit”. Moscow; 2005. p. 87–90. (in Russian).
  4. Grusha YO. Ophthalmologic features of the reconstruction of the orbital region. Proceedings of the 2nd International Congress on Plastic, Reconstructive and Aesthetic Surgery. Moscow; 2001. p. 68. (in Russian).
  5. Gundorova RA, Verigo EN, Chernookov VA. Indications for removal of the eyeball on the basis of posttraumatic scurvy flow analysis. Proceedings of the Inflammatory diseases of the organ of vision Conference; 2004; Chelyabinsk, Russia. Chelyabinsk; 2004. p. 95–8. (in Russian).
  6. Gundorova RA, Neroev VV, Kalashnikov VV. Eye injuries. Moscow; 2009. p. 361–77. (in Russian).
  7. Krasvidi TA, Tychina NP, Narovchenko TN. Main causes of the eyeball removal. Proceedings of the 2nd International ophthalmology conference; 2004 Sept 8–10; Odessa, Ukraine. Odessa; 2004. p. 147–8. (in Russian).
  8. Krasnovid TA. Eye traumatism in modern conditions. Urgent assistance in Ukraine. Proceedings of the Scientific and Practical Conference of ophthalmologists in Chernihiv, Kiev and other regions; 2013; Chernigov, Ukraine. Chernigov; 2013. p. 40–4. (in Russian).
  9. Maletsky AP, Chebotarev EP. [The method of forming the musculoskeletal stump after eyeball evisceration and fixing the eye prosthesis]. Journal of Ophthalmology. 2006;2:31–5. (in Russian).
  10. Maletsky AP, Bushuyeva NM, Chebotaryov EP, inventors; The Filatov Institute of Eye Diseases and Tissue Therapy, assignee. Model of facial denture. Ukr. Patent 11682. 2006 Jan 16.
  11. Maletsky AP, inventor; Maletsky AP, assignee. The method of forming the musculoskeletal stump after eyeball evisceration due to injuries and slow uveitis. Ukr. Patent 46980. 2010 Jan 11.
  12. Maletskiy AP, Dubkova VI, Mayevskaya OI, Bigun NM. [The use of polymer-composite material during the formation of the locomotor stump after evisceration eyes (Preliminary results)]. Journal of Ophthalmology. 2013;6:57–61. (in Russian).
  13. Klapper SR, Mawn L, Brownstein S, Punja K. Abscess formation within a synthetic hydroxyapatite orbital implant. Can. J. Ophthalmol. 1998;33(6):329–32.
  14. Jordan DR, Klapper SR. Wrapping hydroxyapatite implants. Ophthalmic. Surg. Lasers. 1999;30(5):403–7.
  15. Jordan DR, Chan S, Mawn L, Gilberg S, Dean T, Brownstein S, Hill VE. Complications associated with pegging hydroxyapatite orbital implants. Ophthalmology. 1999;106(3): 505–12.
  16. Kirby DB. Enucleation of the eyeball wich immediate implantation into Tenon’s capsule. Trans. Amer. Acad. Ophthalm., Otolaryng. 1945: (Mar.–Apr.):229–35.

Received: 25 May 2018

Published: September 2018

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