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The possibility of metabolic correction of pathological disorders in induced keratitis in animals with dry eye syndrome

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Rafalyuk S.Y.
Danylo Halytsky Lviv National Medical University, Lviv, Ukraine


SUMMARY

Introduction. Keratitis in patients with dry eye syndrome is well-known and not easy problem in ophthalmology.

Purpose. To explore the possibility of correction of metabolic disorders in the cornea during endotoxin-induced keratitis (EK) in animals with dry eye syndrome (DES).

Methods. Experiments were provided on Chinchilla rabbits, which were divided into 3 groups according to the tasks.

Results. The use of Lipoflavon (quercetin) under development of EK in animals with DES has a strong positive effect on the degree of inflammatory signs in the cornea, which were most pronounced during the second period of observation (48 hours). Instillation of quercetin reduced the degree of pathochemical violations in the anterior segment of the eye with tissue in animals with dry eye syndrome, and endotoxin-induced keratitis: the reduction of potential of the glutathione system, abnormal inflammatory process in the development of EK in animals with DES are largely unable to adjust without bioflavonoid – quercetin.

Conclusion. The clinical and experimental data revealed that the use of Lipoflavon (quercetin) result in correction of violations of pathochemical sings of EK with DES.

Keywords: keratitis, dry eye syndrome, glutathione, cornea, conjunctiva, tears.

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The intensity of redox processes in the cornea at the experimental conjunctivitis and development of diabetes

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Zhmud T.M.
Vinnitsa national medical university named after M.I. Pirogov, Vinnitsa, Ukraine


SUMMARY

Introduction. Research on the problems of pathological conditions of surface tissues of the eye is of particular relevance.

Aim. To study intensity of the ORP processes in a cornea at an experimental conjunctivitis and development of diabetes mellitus.

Materials and methods. For realization of experiments 52 rabbit were used, that were divided into three groups: the first – control group (8 rabbit), second – an experience group, animals, with an acute conjunctivitis (23 rabbit), is third is an experience group, animals with a conjunctivitis in the conditions of streptozotocin diabetes (21 rabbit). To investigate the tissue taken cornea and tear liquid, in that determined to activity of the redox (ORP) enzymes.

Results. In the conditions of development of experimental conjunctivitis and streptozotocin diabetes the decline of activity of the ORP enzymes is determined in tissue of cornea and increase of activity of oxidoreductases in a tear liquid.

Conclusions. It is set that at an inflammatory process in a conjunctiva in tissue of cornea for animals with streptozotocin diabetes the state of the ORP enzymes is notedly violated. In a tear liquid for animals at development of experimental conjunctivitis and diabetes mellitus the substantial increase of the ORP processes is educed, that testifies to violation to stability of cellular membrane structures.

Keywords: acute conjunctivitis, streptozotocin diabetes, ORP enzymes.

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